TRESK inhibits glutamic acid-induced apoptosis of neurons in spinal cord via mTOR signal pathway

GAO Run-xing; LIN Wen-jing; HE Wan-you; HUANG Zhen-xing; ZHONG Ji-ying; WANG Han-bing; YANG Cheng-xiang; ZHOU Jun

doi:10.3969/j.issn.1001-9448.2017.24.015

Abstract：Objective To investigate the effect of TRESK on glutamic acid -induced apoptosis of neurons in spi-nal cord via mTOR signal pathway .Methods Primary cultured spinal cord neurons of SD rats were collected and cul-tured.Using random number table, the neurons were randomly divided into 4 groups, blank control group (Group C), glutamic acid treatment group ( Group G) , glutamic acid+TRESK siRNA group ( Group Glu+T) and glutamate+TRESK siRNA+mTOR inhibitor ( Group Glu+T+m) .Neurons in Group C received no process;neurons in Group G were trea-ted with glutamic acid (100 μmol/L) for 24 h;neurons in Group Glu+T were treated with glutamic acid (100μmol/L) for 24 h after transfection with TRESK siRNA; and neurons in Group Glu +T+m were pretreated with Rapamycin ( 20 nmol/L) for 20 min and treated with glutamic acid (100μmol/L) for 24 h after transfection with TRESK siRNA .Cell ap-optosis rate, protein expression levels of cleaved Caspase 3 and Bax, mTOR phosphorylation and TRESK mRNA expression level were assessed .Results Compared with Group C , cell activity were significant reduced , while cell apoptosis rate and protein expression of cleaved Caspase 3 and Bax were significant increased in Group G ( P<0.05 ) .Compared with Group G, cell activity was significant reduced , while cell apoptosis rate and protein expression of cleaved Caspase 3 and Bax were significant increased in Group Glu +T and Group Glu+T+m (P<0.05).Compared with Group Glu +T, cell activity was significant reduced , while cell apoptosis rate and protein expression of cleaved Caspase 3 and Bax were signifi-cant increased in Group Glu +T+m (P<0.05).Compared with Group C, neuron TRESK mRNA expression in was sig-nificantly raised in Group G ( P<0.05 ) .Compared with Group G , neuron TRESK mRNA expression was significantly raised in Group Glu+T and Group Glu+T+m (P<0.05).Compared with Group C, neuron mTOR phosphorylation lev-els were significantly increased in the other 3 groups (P<0.05).Compared with Group G, neuron mTOR phosphorylation levels were significantly increased in Group Glu +T ( P<0.05 ) .Conclusion TRESK inhibits glutamic acid -induced apoptosis of neurons in spinal cord via mTOR signal pathway.