Karyotype analysis on the chorionic villus in different types of early pregnancy loss

NIE Qing-wen; HUA Rui; ZHOU Yao; LI Hong

doi:10.3969/j.issn.1001-9448.2017.23.009

Abstract：Objective To analysis the chromosomal karyotype of chorionic villus in early pregnancy loss with or without fetal pole .Methods A retrospective analysis was conducted from January 2012 to June 2016 in Nanfang Hospital of Southern Medical University .A total of 431 patients, who underwent dilation and curettage ( D&C) and cytogenetic tests for early pregnancy losses , were recruited .Embryonic and anembryonic pregnancy loss were diagnosed based on the presence or absence of an embryonic pole during transvaginal sonography , all cases derived from natural conception or in vitro fertilization and intracytoplasmic sperm injection ( IVF/ICSI ) .The composition of chromosomal in two groups were analyzed , as well as patient characteristics .Results Of the 431 samples from early spontaneous abortion , 255 cases (59.2%) were with abnormal karyotype .The frequency of aneuploidy in embryonic group was significantly higher than that in anembryonic group (65.8%versus 47.1%, P=0.000), especially in women under 35 years old.All monosomy X and ninety percent viable autosomal trisomy ( trisomy 13, 18 and 21) were observed in embryonic group , while trisomy (16 and 22) were mostly observed in anembryonic group .Advanced maternal age increased the risk of aneuploidy , but there was no significant difference between ART pregnancy and aneuploidy after adjustment .There was no statistically sig-nificant difference in abortus sex ratio .Conclusion Chromosomal anomaly is closely correlated to embryonic pregnancy , thouhg may not be the major reason of anembryonic pregnancy .Compared with natural conception , ART doesn′t increase the rate of chorionic villus chromosome abnormality in spontaneous abortion during the first trimester .