Effect of caspase -1 inhibitor on rats with pulmonary hypertension induced by monocrotaline
LIU Ying
DONG Wen-peng
CHEN Chang-fu
YANG Yong-chao
YANG Dong-peng
WANG Xiao-wu
Abstract：Objective To study the role of caspase -1 in rats with pulmonary hypertension and to investigate the pathogenesis of pulmonary hypertension .Methods Thirty-six male Sprague-Dawley ( SD) rats were randomly and e-qually divided into 3 groups, control group, MCT group and YVAD group.MCT(60 mg/kg) was intraperitoneally injected in MCT group , while MCT plus AC-YVAD-CMK was given in YVAD group .Meanwhile , normal saline was given as placebo in control group.Tissue samples were taken on the 21st day.The Right ventricular systolic pressure (RVSP) and right ventricular hypertrophy index [ RV/( LV+S ) ] were measured and compared among all groups .HE staining was used to observe the morphological changes .The expression of caspase -1 and IL-1βwere detected by immunohistochem-istry.Results Compared with control group , RVSP and RV/( LV+S) were significantly increased in MCT and YVAD groups (P<0.05).While comparing with MCT group, they were significantly reduced in YVAD group (P<0.05).His-topathological examination showed that AC -YVAD-CMK could reduce the pulmonary arterial hyperplasia in PAH rats and reduce the infiltration of inflammatory cells in lung tissues .And immunohistochemistry showed that AC -YVAD-CMK reduced the expression of caspase -1 and IL-1β.Conclusion AC-YVAD-CMK can inhibit the activation of caspase-1, reduce the secretion of IL -1β, and then reverse the occurrence and development of pulmonary hypertension .
Keywords：pulmonary arterial hypertensioncaspase-1 inhibitorIL-1β
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 3566-3570 )