Experimental study on the effect of chronic stress damage on serum tumor markers

GUO Wei; SUN Qi; GUI Mu-wei; JIANG Dong

doi:10.3969/j.issn.1001-9448.2017.23.003

Abstract：Objective To investigate the effect of chronic stress damage on serum tumor markers in rat model . Methods Twenty Wistar rats were selected and randomly divided into two groups , model group and blank group .The rats in the both groups were routinely fed , while the model group accepted corresponding chronic stress on the basis of con-ventional feed.Serum samples were collected on the 28th day.Elisa method was used to test serum CEA , CA199, CA724 and AFP.The difference in serum tumor markers between the two groups was compared , and the correlation analysis on serum tumor markers and chronic stress was also conducted .Results The CEA, CA199, CA724 and AFP in blank group were 9.07 ±1.43, 321.01 ±35.95, 8.43 ±1.02 and 7.20 ±0.80, respectively; and were 22.73 ±1.6, 729.11 ± 57.68, 18.66 ±1.11 and 1.11 ±0.89, respectively, in model group.There were significant differences in serum tumor markers between the two groups (P<0.01).The best regression equation was Y=0.199+0.029CA724+0.001CA199+0.045AFP (F=418.70, P<0.01), with the multiple correlation coefficient R of 0.994 and determination coefficient R2 of 0.998.The results suggested that CA199, CA724, AFP were associated with chronic stress, and AFP has the highest correlation with chronic stress .Conclusion Chronic stress damage can stimulate the increase of rats serum tumor mark-ers, suggesting induction of tumorigenesis .