The expression of microRNA-146a and the correlation analysis about synthesis and secretion of IL-6 in lipopo- lysaccharide-induced distal pulmonary artery smooth muscle cells of rats with COPD
YANG Dan
WANG Chang-ming
MA Li-bing
LIN Wu-zhou
XU Qing
CHEN Feng
LIU Xiao-li
LV Qian
JIANG Ming
Abstract：Objective By analyzing the change of microRNA -146a (miR-146a) expression in lipopolysaccha-ride (LPS)-stimulated distal pulmonary artery smooth muscle cells (PASMCs) isolated from rats with chronic obstructive pulmonary disease ( COPD) in different times and to investigate the role and mechanism of microRNA -146a on the in-flammatory response in the PASMCs .Methods The model of COPD was set up with uniting the enzyme digestion and tissue adherent method.The fourth generation of PASMCs were divided into control group and LPS groups .In LPS groups, the cells were treated with LPS (final concentration of 1 μg/mL) and incubated for 12 hours (12 h), 24 hours (24 h), 48 hours (48 h) and 72 hours (72 h), respectively.The interleukin-6 (IL-6) in the supernatant was assessed by u-sing enzyme-linked immunosorbent assay ( ELISA) .And real-time PCR ( Taqman probe method ) was used to assess microRNA-146a expression.Statistical analysis was carried out by Student′s t-test using SPSS18.0 software package. Results Compared with the control group , the expression of microRNA -146a in PASMCs was increased after LPS in-duced in time -dependent manner .The expression of microRNA -146a began to rise when induced for 12 hours, and reached the peak at the 72 hours (P<0.01).And so was the expression of IL -6 in the supernatant (P<0.01).Signif-icantly positive correlation between them was also revealed (P=0.00).Conclusion The IL-6 is increased with LPS stimulated in COPD distal PAMSCs , and so as the microRNA -146a.They have a positive correlation , suggesting mi-croRNA-146a is involved in regulating the inflammatory response of PAMSCs .
Keywords：pulmonary artery smooth muscle cellsmicroRNA-146ainterleukin -6lipopolysaccharidein-flammatory response
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3554-3557 )