Brain magnetic resonance spectroscopy of Fmr1 knockout mice
GUO Yi
CHEN Sheng-qiang
HUANG Yong
TAN Li-lian
PENG Tu-kang
LIN Qiu-xi
Abstract：Objective To investigate the changes of metabolites in brain of Fmr 1 knockout mice ( KO mice) by 1H-MR spectroscopy (1H-MRS) and its application in diagnosis of Fragile X Syndrome (FXS).Methods 1H-MRS was performed on 23 KO mice (experimental group) and 16 wild type mice (control group) at the age of 4 months. The sub-peak areas of N-aeetyl aspartate (NAA), choline (Cho) and creatine (Cr) in the cerebral codex and hippo-campus were measured , and the NAA/Cr and Cho/Cr ratios were calculated and compared by two -sample t-test.Re-sults Compared with control group , the NAA/Cr ratio was significantly reduced (t=-5.912, P<0.01), and the Cho/Cr ratio was significantly increased ( t=3.888, P<0.01) in KO mice.Conclusion 1 H-MRS examination can quanti-tatively assess abnormal metabolites in the brains of KO mice , which may provide important clues for the diagnosis of FXS and evaluation of therapeutic efficacy .
Keywords：Fragile X SyndromeFmr1 knockout miceMagnetic resonance spectroscopy
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3393-3396 )