The Effect of Sterol Regulatory Element-binding Protein-1 on Angiotensin Ⅱ-induced Glomerular Fibrosis
LI Hai-jian
CHENG Gen-yang
LIU Dong
XIAO Jing
TAO Ya-fei
LIU Hui
ZHAO Zhan-zheng
Abstract：Objective To investigate the role of sterol regulatory element -binding protein-1 (SREBP-1) in angiotensin Ⅱ( Ang-Ⅱ) -induced glomerular fibrosis .Methods HMC cells at Passage 3 were applied .The time course and dose course of Ang Ⅱ-induced SREBP -1 activation were defined .The HMC Cells were divided into 4 groups, control group, group of Ang -Ⅱ, group of Ang-Ⅱ+losartan, and group of Ang -Ⅱ+fatostatin.RT-PCR and Western blot were used to assess the expression of CTGF , Collagen Ⅲ, TGF-β1 , Fibronectin and SREBP-1.Re-sults The data showed that Ang -II activated SREBP-1 in the best concentration of 100nmol/L, and action time of 3 hours.Compared to the control group, the expression of CTGF, COL Ⅲ and FN were significantly increased in group of Ang-Ⅱ(P<0.05).Compared to the group of Ang -Ⅱ, the expression of CTGF, COLⅢ, TGF-β1, FN and SREBP-1 was significantly inhibited in the groups of Ang -Ⅱ+losartan and Ang-Ⅱ+fatostatin ( P<0.05 ) .Conclusion Ang-II activates SCAP/SREBP pathway via AT1 receptor and enhances TGF -β1 induced renal fibrosis .
Keywords：AngiotensinⅡsterol regulatory element -binding protein-1transforming growth factor -β1glo-merular fibrosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3239-3242 )