Induced expansion of synovial antigen specific regulatory T cells
HE Xiao-hong
ZHOU Hai -tao
HUANG Qing-chun
Abstract：Objective To amplify the production of synovium specific regulatory T cells in mice (sTregs), and to provide experimental basis for exploring the therapeutic effect of sTregs on Rheumatoid arthritis ( RA) .Methods Mouse bone marrow derived DCs were induced in vitro and loaded with type Ⅱcollagen peptide , subsequently co-cultured with mouse spleen CD4 +CD25 +nTregs under the synergistic action of IL -2 to stimulate the production of synovial antigen sTregs.The inhibition effect of sTregs was evaluated by mixed lymphocyte inhibition assay .Results After the stimulation of DCs for a week, mouse CD4 +CD25 +Tregs were amplified by 6 times, and the proliferating cells (sTregs) showed sig-nificantly stronger inhibition in mixed lymphocyte inhibition test than Poly -Tregs (P<0.01).Conclusion The Synovi-al antigen sTregs could be induced by the DCs loaded with synovial antigen ( TypeⅡcollagen peptide ) from mouse spleen CD4 +CD25+nTregs.
Keywords：Type ⅡCollagenRegulatory T CellsAntigen SpecificityDendritic Cells
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 2907-2912 )