Effect of Shenmai on the expression of glucocorticoid receptor in asthma rats.

SHEN Lu; ZHANG Chun-yun; LIN Chun -yi

doi:10.3969/j.issn.1001-9448.2017.17.001

Abstract：Objective To investigate the therapeutic effect of Shenmai on Asthma rats , and on the expression of glucocorticoid receptor ( GR) in lung of asthma rats .Methods Thirty SD rats were divided into control group , asthma group, glucocorticoid group , low-dose Shenmai group , and high-dose Shenmai group .Each group was sensitized and challenged by ovalbumin except for control group .Dexamethasone was given in Glucocorticoid group , dexamethasone plus Shenmai injections (1ml of low dose group and 2ml of high dose group ) were given in Shenmai groups .The ratio of eosin-ophils (Eos%) and cytokines levels in bronchoalveolar lavage fluid (BALF) were compared.GR mRNA expression in lungs was measured by reverse transcriptase , and GR protein expression was measured by western blot .Results Eos%in BALF of asthma group was significantly higher than control goup , glucocorticoid group and Shenmai groups ( P <0.01).Eos%in BALF of high-dose Shenmai group was significantly lower than glucocorticoid group (P<0.05).IFN-γof asthma group was significantly lower than control goup , glucocorticoid group and Shenmai groups (P<0.05).IL-4 of asthma group was significantly higher than control goup , glucocorticoid group and Shenmai groups ( P<0.05 ) . IFN-γof high-dose Shenmai group was significantly higher than glucocorticoid group ( P<0.05) .IL-4 of high-dose Shenmai group was significantly lower than glucocorticoid group (P<0.05).The expression of GRmRNA in the glucocor-ticoid group was significantly lower than control group ( P<0.05 ) .The expression of GRmRNA in high -dose Shenmai group was significantly higher than glucocorticoid group ( P<0.05) .The expression of GR protein in high -dose Shenmai group was significantly higher than astham group and glucocorticoid group ( P<0.05 ) .Conclusion Shenmai can in-crease the expression of GR mRNA and protein in lung in rats with asthma , which may be one of the main reason of trea-ting asthma.