The role of ASPP2 regulation and endoplasmic reticulum stress in renal injury in aged rats and their correlation.
YANG Song-Hao
ZHANG Hui
YANG An-ning
HOU Miao-miao
ZHU Guang-rong
WANG Nan
XIE Lin
DING Ning
JIANG Yuan-xu
TIAN Jue
YANG Xiao-ling
JIANG Yi-deng.
Abstract：Objective To investigate the role of ASPP2 (one of P53 apoptosis stimulating protein family members) protein and endoplasmic reticulum stress in the kidney injury in aged rats.Methods The 6 months SD rats were assigned into adult group, and those of 18 months in elderly group.Hematoxylin eosin staining (HE) was used to observe the changes of renal structure.And mRNA expression levels of CHOP, ATF6, XBP-1 and GRP78 were detected by qRT-PCR method.Western blot was used to detect the protein expression level of ASPP2.The correlations between the protein expression of ASPP2 and endoplasmic reticulum stress related index CHOP, ATF6, XBP-1 and GRP78 mRNA expression levels were analyzed.Results Compared with adult group SD rats, kidney structure in elderly group SD rats was obviously changed, with significant reduction in CHOP, ATF6, XBP-1 and GRP78 mRNA expression and ASPP2 protein expression levels.The level of ASPP2 was positively correlated with CHOP and XBP-1, and negatively correlated with ATF6.Conclusion In elderly SD rats, the regulation of endoplasmic reticulum stress by ASPP2 may play important role in renal injury.
Keywords：ASPP2endoplasmic reticulum stressaged ratskidney
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2428-2431 )