Correlation between controlled pulmonary collapse and early lung injury in dogs

YAN Xiao; TANG Jian-jun; MA Xian-feng; XIAO Ming; YANG Xue-ying; LU Xin; LIU Gao-wang; LIU Xiao-jun; GU Miao-ning; XIAO Jin-fang

doi:10.3969/j.issn.1001-9448.2017.15.003

Abstract：Objective Using a new type of pulmonary isolation ventilation tool to investigate the correlation between the controlled pulmonary collapse and the early lung injury in dogs.Methods Eighteen dogs were randomly and equally divided into 3 groups (G1-G3).A new single-lumen endotracheal tube was intubated after general anesthesia.Two lung ventilation (TLV) was performed for 20min, and the left one-lung ventilation (OLV) was subsequently carried out with the right lung collapse at 100% (G1), 90% (G2), and 50% (G3).Blood samples were taken from the femoral artery and jugular vein before OLV (T0), and 30min (T1), 60min (T2) and 120min (T3) after OLV.The blood gas analysis was performed assessment of pulmonary shunt rate (QS/Qt).ELISA was applied assessment of serum TNF-α and IL-6.The blood pressure, heart rate, pulmonary artery pressure and et al.were continuously monitored during operation.Lung tissues were collected at T3 to determine the wet/dry weight ratio (W/D).The histopathological changes of the lung tissues were observed by microscopy and performed by blind scoring.Results During TLV, there was no significant difference in all physiological indexes among all 3 groups (P>0.05).During OLV, the mean pulmonary arterial pressures (MPAP) in G2 and G3 were significantly more stable than that in G1 (P<0.05).The QS/Qt, W/D, TNF-α and IL-6 contents in G2 and G3 were significantly lower than those in the G1 (P<0.05).The pulmonary edema, hemorrhage, inflammatory cell infiltration and lung injury scores were also reduced.There was no significant difference between G2 and G3 at each time point (P>0.05).Conclusion Compared with complete pulmonary collapse (100%), controlled pulmonary collapse (90% and 50% collapse) by using the new single-lumen isolation endotracheal tube can alleviate the intraoperative lung injury.There is no significant difference between 90% and 50%.