Effects of advanced oxidative protein products on the apoptosis of rat alveolar osteoblasts
GUO Yuan
ZHANG Chi
LIU Song
WANG Zhao
PAN Xing-fei
WANG Le
Abstract：Objective To investigate the mechanism of advanced oxidative protein product (AOPP)-induced apoptosis of alveolar osteoblasts in rats.Methods AOPP were used to stimulate rat alveolar bone cells.The cell apoptosis ratio was evaluated by flow cytometry.The reactive oxygen species (ROS) produced by the alveolar osteoblasts were measured by ELISA.Western blot was used to detect the expression of the apoptosis related proteins in the alveolar osteoblasts, including Bcl-2 and Bax.Results The primary alveolar bone cells were shown osteogenic-like potential.AOPP induced production of large amount of ROS in alveolar bone cells and resulted in cell apoptosis.The cell apoptosis ratio was significantly increased with the increase of AOPP (P<0.05).The expression of Bcl-2 was down-regulated and the expression of Bax was up-regulated.On the other hand, the cell apoptosis ratio was significantly increased with the prolonging time of AOPP (P<0.05).When the concentration of AOPP reach at 200 μg/mL or the stimulating duration reached at 24 h, the apoptosis rate was the highest.Conclusion Oxidative stress can cause alveolar osteoblasts apoptosis, and causes alveolar bone absorption in the oral chronic inflammatory disease.
Keywords：oxidative stressadvanced oxidative protein productsalveolar osteoblastsapoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2261-2264 )