A study on expression and distribution of collagen in rheumatic heart disease with atrial fibrillation
ZHOU Dong-dong
JIANG Yi-yao
YUAN Chao
SHI Chao
Abstract：Objective To investigate the expression and distribution of collagens in patients with rheumatic valvular heart disease.Methods Thirty-three patients with rheumatic heart disease, who accepted valve surgery, were divided into sinus rhythm group and atrial fibrillation group.Right atrial appendage tissues were obtained during operation.Functional analysis of differentially expressed genes was performed by transcriptomics.By RT-PCR, we detected the expression of type I-IV collagen mRNA in myocardial tissue.The distribution of collagen in myocardium was observed by immunohistochemistry.Results Compared to sinus rhythm group, the left atrial diameter was significantly expanded.Transcriptomics showed that COL1A2, COL2A1, COL3A1 and COL4A3 interacted with COL5A2, COL6A3, COL11A1, and various cytokines.The results of RT-PCR showed that COL1A2, COL2A1, COL3A1, and COL4A3 were up-regulated in atrial fibrillation group.Furthermore, COL3A1 and COL4A3 were significantly up-regulated (P<0.05).Positive type Ⅰ collagen staining was predominantly on the basement membrane beneath the vascular endothelial and partially in the intercellular space.Myocardial collagen fiber was stained with type Ⅱ collagen.Type Ⅲ collagen was positive on capillary endothelial and vascular basement membrane.Type Ⅳ collagen was detected around myocyte, lipocyte, vascular smooth muscle and the vascular endothelial basement membrane.Conclusion Abnormal expression of COL3A1 and COL4A3 is revealed in atrial myocytes, interacting with various cytokines.It is related with the occurrence and development of atrial fibrillation.
Keywords：rheumatic heart diseaseatrial fibrillationcollagen
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2147-2150 )