The regulatory mechanism of CaOx crystal-induced TJs injury and TRPV5 in renal tubular epithelial cells
LU Sui-lin
ZHONG Wen
LI Shu-jue
ZHAO Zhi-jian
Abstract：Objective To investigate the regulatory mechanism of CaOx crystal induced tight junctions(TJs) injury and TRPV5 in renal tubular epithelial cells.Methods Calcium oxalate monohydrate (COM) exposure was performed on canine renal tubular epithelial cells (MDCK) and HEK-293 cells with stable over-expression of TRPV5.EGTA was administrated for TJs injury model.Western blot, immunofluorescence, polarizing microscope and other indicators were applied to assess TJs protein, Na+-K+-ATPase, TRPV5 and crystal adhesion etc.Results COM significantly down-regulated Occludin and ZO-1 protein expression in MDCK cells (P<0.01).The TJs injury significantly increased the adhesion ability of COM to MDCK cells (P<0.05).The expression of Na+-K+-ATPase induced TJs recovery and reached the highest level at 1min.COM inhibited the expression of TRPV5 membrane protein and total protein in HEK-293 cells (P<0.01).Conclusion CaOx can induce the TJs injury in renal tubular epithelial cells, and promote the adhesion of CaOx crystals.When the cell olarity is destroyed, the TRPV5 membrane protein gets transportation problem and thus the expression of TRPV5 membrane protein ecreases.With the increase of urinary calcium, the negative feedback from Na+-K+-ATPase stimulates the repairmen of TJs.With the self-repairmen of TJs, CaOx crystal adhesion to cells decreases.
Keywords：calcium oxalate crystalrenal tubular epithelialtight junctioncrystal adhesionTRPV5
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 2109-2113 )