Effect of transection of the cervical sympathetic trunk on T-lymphocyte subsets, IL-2 and IL-6 in type 2 diabetic rats during perioperative period

LU Yi-mei; XU Wei-li; YANG Zhi-lan; ZHOU Zhi-dong; LANG Hai-li; XU Guo-hai; CHEN Yong; CAI Jun-ying

doi:10.3969/j.issn.1001-9448.2017.14.001

Abstract：Objective To investigate the effect of transection of the cervical sympathetic trunk (TCST) on T-lymphocyte subsets, IL-2 and IL-6 in type 2 diabetic rats during perioperative period.Methods Pathogen-free male Sprague-Dawley rats, aged 10~12 weeks, weighing 250~280 g, were studied.Type 2 diabetic rats were induced by a combination of high-fat and high-glucose diet with intraperitioneal injection of streptozotocin (STZ).Ninety diabetic rats were randomly divided into 3 groups (n=20), diabetes mellitus group (Group D), diabetes mellitus plus exploratory laparotomy group (Group DO) and diabetes mellitus plus TCST plus exploratory laparotomy group (Group DTO).The right side of cervical sympathetic trunk was transected 1 week after the model of diabetes successfully induced in group DTO.The right side of cervical sympathetic trunk was only exposed but not cut in Group D and DO.Exploratory laparotomy was performed 1 week after the model of TCST successfully established in Group DO and DTO.Blood samples were obtained from each group before skin incision (T0), at the end of operation (T1), and 1 day (T2), 3 days (T3) and 7 days (T4) after operation, for determination of blood glucose.And plasma concentration of noradrenaline (NE), interleukin-2(IL-2), interleukin-6(IL-6), the percentages of T lymphocyte subsets (CD3+, CD4+, CD8+) were detected with flow cytometry, as the CD4+/CD8+ ratio was calculated.Results Compared with Group D, the blood glucose, the plasma concentrations of NE and IL-6 in Group DO at T1 to T4 were significantly increased, while the percentages of CD3+ and CD4+ T cells, the CD4+/CD8+ ratio and the plasma concentration of IL-2 were significantly decreased (P<0.05).Compared with Group DO, the blood glucose, the plasma concentration of NE and IL-6 in Groups DTO at T1 to T4 were significantly reduced, while the percentages of CD3+ and CD4+ T cells, the CD4+/CD8+ ratio and the plasma concentration of IL-2 was significantly increased (P<0.05).Conclusion Transection of the cervical sympathetic trunk increase the percentages of CD3+ and CD4+T cells, CD4+/CD8+ ratio and the plasma concentration of IL-2, which can improve the cell-mediated immune function in type 2 diabetic rats.