Study on gene polymorphisms of GSTP1 Ile105Val by di-allele-specific-amplification with artificially modified primers combined with SYBR Green I real-time PCR

LI Shu-bo; LIAO Zhang-xiu; LUO Ying; HE Shan

doi:10.3969/j.issn.1001-9448.2017.13.014

Abstract：Objective To establish a method of di-allele-specific-amplification with artificially modified primers combined with SYBR Green I real-time PCR for gene polymorphisms analysis of GSTP1 codon 105, and to investigate hereditary character of glutathione S-transferase P1 (GSTP1) codon 105 among Guangxi Baise population by this method.Methods Gene polymorphisms of GSTP1 codon 105 were detected by di-allele-specific-amplification with artificially modified primers combined with SYBR Green I real-time PCR in 211 healthy Guangxi Baise people.The genotype frequency of GSTP1 codon 105 in Guangxi population was compared with other groups in China, other countries and regions.Results The GSTP1 Ile105Val genotypes detected by di-allele-specific-amplification with artificially modified primers combined with SYBR Green I real-time PCR were consisted with those detected by sequencing analysis of PCR products.The frequency of allele A in GSTP1 gene Ile105Val was both high in female and male populations (79.7% and 84.5%, respectively).The prevalence rates of GSTP1 Ile105Val genotypes AA, AG and GG were 62.2%, 35.1%, and 2.7% in Guangxi Baise female population, and were 71.0%, 27.0% and 2.0%, respectively, in males.There was no significant difference between female and male populations in allele or genotype frequency of GSTP1 Ile105Val among Guangxi Baise population.The distribution frequency of genotype in Guangxi Baise population had no significant difference with Liaoning, Ningxia Hui Ethnicity, India or Russian population, but significantly differed with other groups in Xinjiang Uighur, Mongol nationalities, Korean nationality, and Caucasian women (P<0.01).Conclusion Di-allele-specific-amplification with artificially modified primers combined with SYBR Green I real-time PCR is an easy, rapid, and accurate method for detection of gene polymorphisms of GSTP1.Gene polymorphism of GSTP1 in codon 105 has no gender difference, and is different from those in other regions of China and other countries.