Effect of 2-hydroxyglutarate on cell proliferation and migration of human glioblastoma cells
LU Jie
YU Lei
LI De-pei
QI Song-tao
Abstract：Objective To investigate the effect of 2-hydroxyglutarate (2-HG) on proliferation and migration of isocitrate dehydrogenase (IDH) wild-type glioma.Methods The wild-type glioma cells were cultured.CCK8 method was used to detect the inhibiting effects of different concentrations of 2-HG (5, 10, 15, 20, 30, 40 mmol/L) on the proliferation of glioma cells.Cell migration test was perforned to detect the migration of glioma cells.And PCR was carried out to detect the expression of mRNA of related genes.Results Exogenous treatment of 2-HG transformed the morphology of glioblastoma cells in concentration-dependent manner.Exogenous treatment of 2-HG promoted the abilities of proliferation and migration, down-regulated the epithelial cell marker E-cadherin, and up-regulated the mesenchymal cell marker vimentin and β-catenin.Conclusion 2-HG can promote the proliferation and migration of IDH wild-type glioblastoma cells.
Keywords：2-HGglioblastomaIDHproliferationmigration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1953-1956 )