The correlations between the expression of HBP and CD64 and different bacterial infections in the acute exacerbation of chronic obstructive pulmonary disease

ZHANG Chun-yun; HE Yao-jun; JIANG Yan-qiong

doi:10.3969/j.issn.1001-9448.2017.12.012

Abstract：Objective To observe the levels of heparin-binding protein (HBP) and CD64 in the acute exacerbation of chronic obstructive pulmonary disease (AECOPD) with different bacterial infections, and to investigate the correlations between them and the bacterial infection, also the correlation between HBP and cTnI.Methods The level of HBP in AECOPD patients with bacterial infection (n=100) and non-bacterial infection (n=80), and in 80 healthy persons was determined by ELISA method, as the index of CD64 was determined by flow cytometry.The level of cTnI was determined by automatic immunoassay in bacterial infection cases.Bacterial infection was defined as patients with bacteria concentration exceeded 107cfu/mL.Results The levels of HBP and CD64 in the bacterial infection group were significantly higher than those with non-bacterial infection [HBP: (86.8±27.39)ng/mL vs.(29.35±7.54)ng/mL;CD64: 6.28±2.23 vs.0.69±0.19] and health group [HBP: (16.08±4.45)ng/mL;CD64 index 0.47±0.12] (F=960.97, P<0.01;F=108.43, P=0.01).And there was no significant difference in the level of HBP between non-bacterial infection and health group (P>0.05), but the difference in the level of CD64 between the two groups was significant (P<0.05).The level of HBP in gram positive bacterial infection was significantly higher than that in gram negative bacterial infection [(95.88±11.61)ng/mL vs.(74.32±17.84)ng/mL] (t=6.795, P<0.01).The plasma level of HBP in bacterial infection was significantly positively correlated with cTnI (r=0.904).Conclusion HBP and CD64 can be used as indicators for the diagnosis of bacterial infection in patients with AECOPD.We can determine the type of bacterial infection and the effect of infection on cardiac function in patients with AECOPD according to the plasma level of HBP.