Genotyping of mycobacterium tuberculosis from Guangdong province by mycobacterial interspersed repetitive units and RD105 deletion
HUANG Xin-chun
WEI Wen-jing
LI Hai-cheng
ZHAO Jiao
GUO Hui-xin
WU Zhu-hua
LI Zhen-yan
SUN Qi
ZHANG Chen-chen
CHEN Tao
ZHOU Lin
Abstract：Objective To understand the dynamic features of mycobacterium tuberculosis (MTB) in population and distribution in Guangdong province.Methods Mycobacterial interspersed repetitive units-24 (MIRU-24) were analyzed on 455 MTB patients with MTB in Guangdong in 2013, so as to develop an optimal MIRU for M.tuberculosis typing.The 173 patients with MTB in 2009, 455 patients in 2013, and 225 patients in 2015 were analyzed by that optimal MIRU set.RD105 deletion test was applied to distinguish Beijing and Non-Beijing strains.Drug susceptibility test was done by proportion method.Gene clustering was analyzed using BioNumerics (7.6).The statistical analysis used chi-square test.Results The rate of Beijing strains in 2015 (70.2%) was higher than those in 2009 (59.5%) and 2013 (59.3%).At the same time, this rate was the highest in midland (71.3%) and the lowest in east (55.0%).There was no significant difference in multidrug-resistant (X2=2.176, P=0.337) or extensively drug-resistant tuberculosis (X2=1.468, P=0.480) among different years in Guangdong province.There were differences in the polymorphisms of MIRU-24.The resolution capability of MIRU-12 (HGI=0.999 1) was similar with MIRU-15 (HGI=0.999 2) and MIRU-8 (HGI=0.998 5).The minimum spanning tree (MST) of 853 M.tuberculosis cases was mainly comprised of 1 Beijing strain and 2 Non-Beijing strains, which could be divided into Group I and II.The predominant strain in Guangdong was Group II.The rates of Group I strain in 2009 and 2013 were higher than that in 2015, and was higher in east (43.069%) than in midland (27.206%).The results of Group II strain were per contra.The MST of Beijing strains was mainly comprised of one complex consisted of 407 unique genotypes and 46 clusters, while the MST of Non-Beijing strains was mainly comprised of 2 complexes consisted of 309 unique genotypes and 6 clusters.Conclusion Beijing strains are the most predominant strains in Guangdong province and there are a rising trend over time.The rate of Beijing strains in midland is the highest.The trend of MDR and XDR in Guangdong is stable.The study of molecular epidemiology of MTB from Guangdong could achieve satisfactory results by MIRU.
Keywords：mycobacterium tuberculosisRD105 deletionmycobacterial interspersed repetitive units
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1827-1832 )