Effect of miR-143 overexpression on the invasion and migration of osteosarcoma 143B cells
LI Yong
QI Wei
WANG Bao-cang
FAN Chao
Abstract：Objective To study the effect of miR-143 overexpression on the invasion and migration of human osteosarcoma 143B cells.Methods The miR-143 overexpressing vector was carried by lentivirus and transfected into osteosarcoma 143B cells.The experiment was divided into, experimental group (transfected with miRNA-143 overexpression sequence), control group (transfected with negative control-NC sequence), and blank group (PBS).Real-time PCR was used to detect the expression of mRNA.The protein expression was detected by Western blot.Transwell invasion assay was used to detect the invasive ability of the cells.Cell migration was measured by cell scratch test.Results The expression of miR-143 mRNA and protein in the experimental group was significantly higher than control group and blank group (P<0.05).However, the mRNA and protein expression of MMP-13 was significantly down-regulated in experimental group than control group and blank group (P<0.05).The number of transmembrane cells and healing rate of scratches in the experimental group were significantly lower than control group and blank group (P<0.05).Conclusion MiR-143 overexpression inhibits invasion and migration of osteoblast 143B cells by acting on MMP-13 target genes.
Keywords：osteosarcomamicroRNAmatrix metalloproteinase-13cell invasioncell migration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1809-1813 )