The effect of NFATc1 over-expression on zoledronate-induced inhibition of osteoclastogenesis
ZHANG Guang-feng
YIN Lu-jia
QI Meng-chun
DONG Wei
FENG Xiao-jie
WEN Li-ming
SUN Hong
Abstract：Objective To investigate the rescue effect of nuclear factor of activated T cells type c1(NFATc1) over-expression on zoledronate-induced inhibition of osteoclastogenesis.Methods RAW264.7 cells were transfected with mouse recombinant NFATc1 lentivirus, and protein expression of NFATc1 was verified.The cells were divided into Group A and B, in which the cells were transfected with blank vector and recombinant NFATc1 vector, respectively.The cells in both groups were treated with 50 ng/mL RANKL for 5 days and 1×10-6 mmol/mL zoledronate for 48 h from Day 2.The cells were harvested on Day 6 and osteoclastogenesis, as well as gene expression of NFATc1, TRAP and c-Src were examined.Results NFATc1 protein was successfully expressed in RAW264.7 cells.TRAP positive multinuclear osteoclast count, and the number and size of absorption lacunae on dentin slices in Group B were all significantly higher than those in Group A (P<0.01).Protein levels of NFTAc1, TRAP and c-Src in Group B were also significantly higher than those in Group A (P<0.01).And mRNA levels of above three genes in Group B were significantly higher than those in Group A (P<0.01).Similar results were also achieved in immunofluorescent cytochemistry examination.Conclusion Over-expression of NFATc1 can rescue zoledronate-induced inhibition of osteoclastogenesis and up-regulate gene expression of NFATc1, TRAP and c-Src.As a key transducer of Ca2+ signal, NFATc1 may play a key role in zoledronate-induced inhibition of osteoclastogenesis.
Keywords：nuclear factor of activated T cells type c1gene recombinanationzoledronateosteoclasttartrate-resistant acid phosphotase
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1795-1799 )