Effects of dexmedetomidine on inflammatory cytokines, S100β and NSE in rats undergoing cardiopulmonary bypass

CHEN Yan-hua; ZHANG Bing-dong; WEI Yi; ZHOU Li-fang; MAO Qi; XIE Yu-bo

Abstract：Objective To evaluate the effects of dexmedetomidine (Dex) on inflammatory cytokines,S100β and NSE in rats undergoing cardiopulmonary bypass.Methods Forty Sprague-Dawley rats,weighing 250 ～350 g,were randomly divided into 4 groups (n =10 each) using a random number table,sham operation group (Group S),cardiopulmonary bypass group (Group C),Dex Ⅰ group (Group D Ⅰ),and Dex Ⅱ group (Group D Ⅱ).Rats in the Group G received no CPB procedure.CPB rat model was established in Group C,Group D Ⅰ and Group D Ⅱ.In Group D Ⅰ,Dex was infused at a rate of 2.5 μg/(kg · h) for 2 h during CPB after a loading dose of Dex by 2.5 μg/kg.In group D Ⅱ,Dex was infused at a rate of 5 μg/(kg · h) for 2 h during CPB after a loading dose of Dex by 5 μg/kg.Before CPB (T0),1 h after CPB (T1),and 2 h after CPB (T2),venous blood samples were collected for detection of plasma concentrations of interleukin 6 (IL-6),interleukin 10 (IL-10),S100β and NSE in using ELISA.Results Compared with Group S,concentrations of IL-6,IL-10,S100β and NSE at T1 and T2 were significantly increased in Group C (P ＜ 0.05).Compared with Group C,the concentrations of IL-6 and S100β at T2 were significantly reduced in Group D Ⅰ (P ＜0.05),as the concentrations of IL-6 and S100β at T1 and T2 were significantly decreased in Group D Ⅱ (P ＜ 0.05).Meanwhile,the concentrations of NSE at T1 and T2 were significantly reduced in Group D Ⅰ and Group D Ⅱ (P ＜0.05).Compared with Group D Ⅰ,the concentration of S100β at T1 was significantly reduced in Group D Ⅱ (P ＜ 0.05),and the concentration of NSE at T2 was significantly reduced in Group D Ⅱ (P ＜ 0.05).Conclusion Dex can attenuate the cerebral injury followed by CPB through inhibiting the inflammatory responses.And there is a certain correlation between the effect of cerebral protection and the doses of Dex.