Effect of RNAi silencing GCF2 on the invasion of human hepatocellular carcinoma cell line BEL-7404
GUO Gu-nan
CHEN Jun
XIONG Bin
NING Xiao-mei
LI Fu-jian
PENG Jia-yin
LI Yu-bao
ZHONG Ming-lin
HUANG Zhi-xi
LI Jing-ping
Abstract：Objective To observe the effect of GCF2 on invasion of human hepatocellular carcinoma cell line BEL-7404.Methods The siRNA targeting GCF2 mRNA (named as GCF2-siRNA) was transiently transfected into BEL-7404 cells.Forty-eight hours after transfection, cell proliferation was determined by cell counting Kit-8, and cell invasion ability was determined by Tran-swell invasion assay.MMP-9 and MMP-23 protein expression was assessed by western blotting.Results GCF2-siRNA effectively down-regulated GCF2 expression.Compared with the negative control group and MOCK group, cell proliferation and invasion abilities in siRNA-GCF2 group were remarkably suppressed.Meanwhile, MMP-9 and MMP-23 protein expression was also obviously reduced.Conclusion Down-regulation of GCF2 by siRNA suppresses cell invasion abilities of BEL-7404, which suggests that GCF2 plays a crucial role in the progression of HCC by affecting cell invasion.
Keywords：GCF2human hepatocellular carcinomainvasion
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1485-1488 )