Protective effect of rhubarb on blood spinal cord barrier after spinal cord injury in rats
YUAN Wen-chao
ZHANG Hua
HUANG Gui-cheng
GUO Yang
YUAN Han
WANG Lei
ZHOU Long-yun
MA Yong
Abstract：Objective To evaluate the effects of Rhubarb on blood spinal cord barrier in rats after spinal cord injury (SCI), looking for a best concentration of Rhubarb decoction lavage and to provide a basis for clinical treatment of SCI.Methods A total of 125 adult healthy SD rats were randomly divided into sham operation group (Sham), model group, and low, medium and high dose groups.Rhubarb solution was given in treatment groups, while normal saline as placebo was given in the other 2 groups.The neurological function of hind limbs was recorded using BBB score.The water content in spinal cord was observed.And using fluorescence microscope to observed the blood spinal cord barrier after injection of Evans blue (EB).Results There were significant differences in BBB scores and water content in spinal cord between control group and model group, rhubarb low, medium and high dose groups (P<0.05).Fluorescence microscope showed that there was no EB stain in control group, while rich EB stain was revealed in model group.The EB stain in rhubarb groups showed reduction in different degreed compared with model group, especially in rhubarb medium dose group.Conclusion In the medium dose group, rhubarb can effectively reduce the permeability of the blood spinal cord barrier in rats after SCI.
Keywords：spinal cord injuryrhubarbdecocting concentrationblood spinal cord barrier
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1481-1484 )