Effect of exosomes on the proliferation, apoptosis and migration of A549 cells
WU Wei-dong
YI Yong-sheng
LIU Dan
DING Feng
LUO Lei
ZHOU Yang-yang
Abstract：Objective To observe the effect of lung cancer cell-derived exosomes on the proliferation, apoptosis and migration of tumor cells.Methods The exosomes were gathered from the culture supernatant of A549 lung cancer cells by means of multi-step ultracentrifugation.The morphology of exosomes was observed by transmission electron microscopy.Western blot was used to assess the expression of transmembrane proteins on its surface.MTT assay and ELISA were used to detect the influence of exosomes on the proliferation and apoptosis of A549 cells.By using trans-well, the impact of exosomes on migration capability of A549 cell was observed.Results The extracted exosomes had uniform size with a diameter of about 30-100 nm, most of them were oval or spherical, coated with membrane, and rich in four-times transmembrane proteins, such as CD9 and CD63.MTT assay suggested that exosomes could promote the proliferation of A549 cells in time-and dose-dependent manners (P=0.000).ELISA assay showed that exosomes inhibited the apoptosis of the tumor cells (F=365.496, P=0.000).Trans-well experiment showed that exosomes promoted migration capacity of A549 cells (F=252.005, P=0.000).Conclusion Lung cancer cell-derived exosomes can promote proliferation and reduce apoptosis of A549 cells, and increase its migration ability.
Keywords：lung cancerexosomesproliferationapoptosismigration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1477-1480 )