The effect of long non-coding RNA PVT1 silencing on K562 cell proliferation
ZHENG Chan-li
LIU Ge-xiu
CHEN Sheng-ting
ZHANG Huan
HE Dong-mei
LI Yang-qiu
Abstract：Objective To screen the effective small interfering RNAs (siRNAs) targeting against long non-coding RNA (lncRNA) PVT1 (plasmacytoma variant translocation 1), and to observe its influence on the proliferation of human leukemic cell K562.Methods Four siRNA sequences targeting against PVT1 (PVT1-siRNA1, 2, 3, 4) and scramble control sequence (SC-siRNA) were designed and synthesized.Using HiperFect transfection reagent, all of the siRNAs were transfected into K562 cells.The experiment was divided into siRNA1 group, siRNA2 group, siRNA3 group, siRNA4 group, SC-siRNA group, idling group and untransfected group.The PVT1 RNA expression level was assessed by real-time RT-PCR.The cell proliferation was determined by CCK8 assay and the cell cycle was detected by Flow cytometry.Results The expression level of PVT1 RNA of K562 cells in siRNA4 group was significantly reduced compared with SC-siRNA group, idling group and untransfected group 24 and 48 hours after transfection (P<0.01), while there was no significant difference among siRNA1 group, siRNA2 group, siRNA3 group and untransfected group (P>0.05).The cell proliferation of K562 cells in the siRNA4 group was significantly inhibited as compared with SC-siRNA group and idling group 24, 48 and 72 hours after transfection (P<0.01).The cells in the siRNA4 group were arrested at G1 phase 48 and 72 hours after transfection (P<0.01).Conclusion PVT1-siRNA can inhibit the proliferation of K562 cells through arresting the cell cycle at G1 phase.
Keywords：long non-coding RNAPVT1small interfering RNAK562 cellscell proliferationcell cycle
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1473-1476 )