The change of cardiac RAGE expression in acute myocardial infarction

KONG Hong-hui; FU Zhen-yang; LIN Ji-jin

doi:10.3969/j.issn.1001-9448.2017.10.001

Abstract：Objective To investigate the change of cardiac RAGE expression level in myocardial infarction rat model.Methods Myocardial infarction rat model was constructed by LAD ligation in Spraguee Dawley rats (230±10)g.The mRNA level of RAGE in infarcted and non-infarcted regions of hearts was assessed by qPCR.The protein expression level was measured by immunohistochemistric and Western Blot analysis, and pERK1/2 levels were also detected by Western Blot analysis.Results The RAGE mRNA level of infracted regions was significantly higher than that in non-infarcted regions (P<0.05).The protein expression of RAGE in infarcted regions was significantly increased comparing with that in non-infarcted regions (P<0.05).The pERK1/2 expression level in infarcted regions was also significantly higher than that in non-infarcted regions (P<0.05).There was no significant difference in RAGE mRNA level, RAGE protein expression level or pERK1/2 expression level between cardiac tissue of Sham-operated group and non-infarcted regions of experimental group (P>0.05).Conclusion RAGE protein is activated in infarcted hearts tissues in rats, which increases the activation of MAPK/ERK1/2, subsequently leads to the activation of downstream cytokines, including NF-κB, finally induces overwhelming inflammatory and myocardial remodeling.