Associations between bone mineral density and genetic polymorphisms of vitamin D receptor gene and calcitonin receptor gene in female Han population in Xinjiang region
MENG De-feng
YANG Li
ZHANG Bin
CHEN Fu-yu
SHI Chen-hui
WANG Wei-shan
Abstract：Objective To investigate the associations of bone mineral density (BMD) with vitamin D receptor (VDR) gene and calcitonin receptor (CTR) gene polymorphisms in Han women from Xinjiang;and to discuss the genetic susceptibility factors of osteoporosis (OP).Methods VDR and CTR genotypes were identified with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), so as to analyze distribution of VDR and CTR polymorphisms of 336 Han women from Xinjiang.BMD was measured by dual energy X-ray absorptiometry (DEXA) at the lumbar spine (L2-4), Ward's triangle, great trochanter, and femoral shaft.Comparing the differences of BMD values of different locations among different genotypes.Results A total of 336 women were included in the study.The distributions of VDR and CTR genotypes met Hardy-Weinberg equilibrium (all P>0.05);and there was no significant difference in VDR or CTR genotype frequency between osteoporosis group (n=90) and non-osteoporosis group (n=246) (P>0.05).We also found that BMD values of different locations were significantly negatively correlated with age (P<0.05) and positively correlated with body mass index (BMI) (P<0.05).When BMD were adjusted by age and BMI, the women with CT genotype of CTR gene had a higher adjusted BMD than CC genotype at Ward's triangle (P<0.05) in non-osteoporosis group.The adjusted BMDs at L2-4 and Ward's triangle were significant lower in the women with Bb genotype of VDR gene compared to the bb genotype (P<0.05).Compared to the bb genotype at great trochanter and femoral neck in non-osteoporosis group, the adjusted BMDs showed a decreasing tendency in women with Bb genotype.The adjusted BMD of CCBb genotype was the lowest.Conclusion Age is closely correlated to the change of BMD.BMI is a protective factor for OP in Han women in Xinjiang, and increasing weight may have a positive effect on preventing and treating osteoporosis.Bb genotype of VDR is associated with lower BMD.Compound CCBb genotype may be regarded as a genetic marker to predict the risk of osteoporosis in Han women in Xinjiang.
Keywords：vitamin D receptorcalcitonin receptorgenetic polymorphismsosteoporosisbone mineral density
Publication Date：2017-01-01
Pages：5( 1343-1347 )