The FAS/FASL single nucleotide polymorphism in Southern China population

XU Yi-ning; YU Cheng-ming; HU Li-li

doi:10.3969/j.issn.1001-9448.2017.09.009

Abstract：Objective To investigate the distribution of FAS and FASL single nucleotide polymorphism (SNP) FAS-1377G/A, FAS-670A/G and FASL-844T/C in southern Chinese people, and compare the distributional differences among different populations.Methods Sequence variations were detected by PCR-RFLP, genotype and allele frequency distribution differences among different ethnicity were tested by chi-square test.Results The frequencies of G and A for FAS-1377G/A were 48.61% and 51.39%, respectively;of A and G for FAS-670A/G were 59.03% and 40.97%, respectively;and of T and C for FASL-844T/C were 27.67% and 72.33%, respectively.The distributions of the 3 different genotypes were concordant with the Hardy-Weinberg equilibrium.Significant differences were observed in the allele frequency of FAS-1377G/A between the Southern China population with the other different populations in China or other countries;so they were of FASL-844T/C between the Southern China population and Indian, Egyptian, Iranian or Tunisian populations (P<0.05).No difference found between the Southern China and any one of the other population in FAS-670A/G.Conclusion The population of Southern China has the FAS/FASL gene polymorphisms FAS-1377G/A, FAS-670A/G and FASL-844T/C.There are significant differences in the allele frequencies of FAS-1377G/A and FASL-844T/C between different populations.