Establishment of ovarian cancer dormancy of nude mouse model based on the in vivo imaging technology
HU Xiang-dan
XIAO Jing
ZHAI Qiu-li
CHEN Xiao-feng
Abstract：Objective To establish ovarian cancer dormancy of nude mouse model based on the in vivo imaging technology, and use the small animals living imaging techniques to verify the existence of the dormant cells.Methods human ovarian cancer skov 3 cells were transfected with firefly luciferase gene via slow virus vector.After that the skov 3 cells with stable expression of firefly luciferase gene (skov-3/luc) were obtained by screening.The skov-3/luc cells were selected and inoculated into nude mice of right axillary region under the skin.The skin tumor was observed on fluorescent and subsequently resected.The nude mice were fed for 8 weeks after the operation, the nude mice with no gross tumor were selected for further observation with fluorescent by ling imaging.The dormant ovarian cancer nude mouse model was established with positive in vivo imaging.Results Stable expression of luc was observed in skov 3/Luc cells.Ovarian cancer dormancy nude mouse model was successfully established based on the living imaging technology and were verified by the small animals living imaging techniques.Conclusion The ovarian cancer dormancy nude mouse model based on living imaging technology is an ideal experimental animal model to study the mechanism of tumor cell dormancy and the ways to maintain and induce tumor cell dormant.
Keywords：live animals imagingovarian cancerdormancy modelsnude mouse
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1305-1308 )