Effects of salvia miltiorrhiza water extract on testicular spermatogenic function and sperm DNA damage in mice

ZHOU Wen; LU Shan; ZHOU Huan-qun; YUAN Qi-long; WANG Ai-ai; NI Yun-ping

doi:10.3969/j.issn.1001-9448.2017.08.006

Abstract：Objective To investigate the effects of Salvia miltiorrhiza water extract on testicular spermatogenic function and sperm DNA damage in mice.Methods Forty Kunming mice were randomly divided into low,medium,and high concentration,control and positive control groups.Mice in low,medium and high concentration groups were given with 1 500 mg/kg,3 000 mg/kg and 6 000 mg/kg salvia miltiorrhiza water extraction by gavage once a day,respectively;as the replaced saline and bolus cyclophosphamide were given to those in control group and positive control group,respec tively.All mice were sacrificed after 30 days,testicular spermatogenic cycle,micronucleus rate and sperm DNA fragmentation were detected by flow cytometry,giemsa staining and chromatin dispersion methods.Results Micronucleus rates of early spermatogenic cells of low,medium and high concentration groups were lower than control group,but with no statistical differences (P ＞ 0.05).With the increase of salvia miltiorrhiza,testicular haploid ratio (1 C) rised gradually,and significant differences were observed between high concentration and control group (P ＜ 0.05).There was no significant difference in testicular diploid (2C),tetraploid (4C),(2C∶ 4C) or (1C∶ 4C) ratio among control,and low,medium,and high concentration groups (P ＞ 0.05).Sperm DNA fragmentation rate decreased gradually among control,and low,medium and high concentration groups,and significant difference was observed between high concentration and control groups (P ＜ 0.05).Conclusion Salvia miltiorrhiza water extract could promote testicular spermatogenic process,reduce spermatogenic chromosome aberration and sperm DNA damage.