Curcumin attenuates H2O2-induced apoptosis and premature senescence in endothelial cells via autophagy up-regulation
CHEN Guo-qin
LI Jin-liang
ZHANG Wen-zhu
SONG Ming-cai
Abstract：Objective To investigate the effects of curcumin (Cur) on autophagy,apoptosis and premature se nescence induced by H2 O2 in human umbilical vein endothelial cells (HUVECs).Methods HUVECs were cultured in vitro and randomized into control group (treated with culture medium),model group (treated with 60 μmoL/L H2O2),Cur group (treated with 60 μmol/L H2O2 and 10 μmol/L Cur) and 3-MA group (treated with 60 μmol/L H2O2,10 μmol/L Cur and 1 mmol/L 3-Methyladenine).After 4 days of culture,DCFH-DA was used to measure the level of reactive oxygen species (ROS) in endothelial cells.Cells apoptosis were detected by flow cytometry.SA-β3-gal staining was used to identify cells aging status.Western blot was conducted to detect the expression of autophagy marking protein LC3 and p62.Results Compared with control group,the level of ROS,apoptosis rate,the number of SA-β-gal positive cells and the expression of p62 protein were significantly increased (P ＜ 0.05) in model group,while the expression of LC3-Ⅱ / Ⅰ protein showed no significant difference (P ＞ 0.05).Compared with model group,the level of ROS,apoptosis rate,the number of SA-β-gal positive cells and the expression of p62 protein were significantly reduced (P ＜ 0.05) in Cur group,and the expression of LC3-Ⅱ / Ⅰ protein was significantly increased (P ＜ 0.05).All the effects of curcumin mentioned above were inhibited by 3-MA,the autophagy inhibitor (P ＜ 0.05).Conclusion Curcumin can attenuate H2 O2-induced apoptosis and premature senescence in endothelial cells via autophagy up-regulation.
Keywords：curcuminendothelial cellsoxidative damageapoptosisautophagysenescence
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1155-1158 )