Establishment of hemorrhagic cardiac arrest model in rabbits

ZHANG Min-hai; ZHENG Ru-gang; JIAN Hong-jian; LIANG Guo-dong; WU Gang

doi:10.3969/j.issn.1001-9448.2017.08.003

Abstract：Objective To establish a rabbit model of hemorrhagic cardiac arrest in rats.Methods Twenty-senven New Zealand rabbits were randomly divided into three groups,sham group (Group A,with no bloodletting or resuscitation),uncontrolled group (Group B,with bloodletting alone),controlled groups (Group C,with bloodletting and resuscitation).No treatment was given to subjects in Group A.An estimated 50％ of total blood was withdrawn from the right jugular vein and the right femoral artery in Group B,and they were maintained at the state of cardiac arrest for 15 minutes.Resuscitation using lactated Ringer's solution (LR) and autotransfusion were performed in Groups C.Arterial blood sample was taken at each time point for blood gas analysis and coagulation test,and fluid requirements,blood loss and survival rate at 6h were recorded.Results After the cardiac arrest,the MAP decreased to 14.5 ±5.3 mmHg.In the 30 min resuscitation,spontaneous circulation had been regained,with the MAP of 69.75 ± 14.37 mmHg,the HR of 185.25 + 12.89 times per min.According to blood gas analysis,the pH value,PaCO2,Hct,Hb,actual bicarbonate,standard bicarbonate,actual base excess,and standard base excess were significantly reduced,and the K + and lactic acid were significantly elevated in the 5 min of resuscitation (Compared with 0 min,P ＜ 0.01).The actual bicarbonate,standard bicarbonate,actual base excess and standard base excess were significantly lower at 25 min of resuscitation than 0 min,except for K+.In the 25 min of resucitation,the calcium value had no difference compared with T0 all the time.Conclusion Through exsanguination from the right jugular vein and the right femoral artery,the hemorrhagic cardiac arrest model of rabbit was established to mimic the clinic situation and lay a foundation for further study of hemorrhagic cardiac arrest.