The correlation between fibroblast activation protein and chronic Hepatitis B-related liver diseases
ZHANG Ye-qiong
ZHANG Ka
SUN Hai-xia
SHU Xin
CAO Hong
LI Gang
Abstract：Objective To analyze the mRNA expression and significance of fibroblast activation protein (FAP) in HBV related hepatocellular carcinoma (HCC),cirrhosis and chronic hepatitis.Methods We collected specimens from 47 HCC patients with surgical resection,29 HBV related liver cirrhosis and 22 chronic hepatitis;and 17 normal liver tissues were also collected as control.Real-time PCR was performed to analyze the expression of FAP mRNA.Enzyme-linked immunosorbent assay was used to measure FAP protein concentrations.Results The mRNA expression of FAP in liver tissues from HCC,cirrhosis,hepatitis and normal controls was 4.73 ± 1.60,1.86 ± 1.04,0.98 ± 0.80 and 1.00 ± 0.00;among which there were significant differences (F =7.156,P ＜ 0.05).Enzyme-linked immunosorbent assay showed that the concentrations of FAP in 1 μg total protein in liver tissues from HCC,cirrhosis,chronic hepatitis and normal controls were (1 288.28 ± 695.82) pg/mL,(1 176.11 ± 677.43) pg/mL,(1 044.58-± 389.48) pg/mL and (848.70 ± 540.74) pg/mL,respectively.Conclusion There are significant differences in the expression of FAP mRNA among hepatitis,cirrhosis,liver cancer and normal control;as the disease progresses,the expression of FAP is progressive.
Keywords：hepatitis B viruscarcinomahepatocellularcirrhosischronic hepatitis Bfibroblast activation protein
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 851-854 )