Diagnostic utility of Multiprobe Fluorescence in situ Hybridization combined with G-banding chromosome analysis in Uyghur Adult Patients with newly diagnosed Acute Myeloid Leukemia in Kashgar region
HUANG Jing
ZHOU Chang-hua
Ayimunisa Abudureheman
GAO Teng-teng
Hairesa Abulimiti
LIU Zhi
Munire Aini
XU Li
Aikebaier Abudoureyimu
Abstract：Objective To explore the clinical application of multiprobe fluorescence in situ hybridization (FISH) combined with conventional cytogenetic G-banding(CCG) analysis in the diagnosis of acute myeloid leukemia (AML) in Uyghur adult patients in Kashgar region.Methods The multiprobe AML panel comprising 8 different FISH probes for PML/RAR transfusion gene,AML1/ETO transfusion gene,CBF/MYH11 transfusion gene,MLL gene,Del(20q),-7/ Del(7q),Del(5q),and P53 deletion was performed in 30 Uyghur adult patients newly diagnosed with AML in Kashgar region.The multiprobe FISH results were compared with those of CCG.Results With the multiprobe FISH panel,cytogcnetic aberrations were detected in 22 cases (22/30,66.7％),which have involved PML/RAR transfusion gene,AML1/ETO transfusion gene,CBF/MYH11 transfusion gene,MLL gene,trisomy 8,-7/Del (7q),Del (5q),P53 deletion.CCG was only idcntificd in 9 cases with the corresponding cytogenetic abnormalities and 2 cases with other cytogenetic abnormalities.The positive rate of multiprobe FISH was significantly higher than the CCG (P ＜ 0.05),and the detection rate by the combination of FISH and CCG increased to 73.3％.Conclusion Comparing with CCG,multiprobe FISH panel is more accurate,effective and rapid in the diagnosis of AML with cytogenetic aberrations.Multiprobe FISH combined with CCG can improve the detection rate of cytogenetic aberrations in AML and can provide more objective and reliable evidences for the diagnosis of Uyghur newly diagnosed adult AML in Kashgar region.
Keywords：multiprobe fluorescence in situ hybridizationcytogentic G-bandingcytogenetic aberrationacute myeloid leukemiaUgyhur
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 692-695 )