The effects of bone marrow mesenchymal stem cells intervention at different times on acute lung injury repairmen

YE Yong-shun; LIAO Hua; LI Wen-feng; WANG Yi; LIU Hua

doi:10.3969/j.issn.1001-9448.2017.05.001

Abstract：Objective To investigate the intervention effects of bone marrow-derived mesenchymal stem cells (BM-MSCs) on the repairmen of acute lung injury (ALI) at different times.Methods The rat BM-MSCs were isolated,cultured and identified in vitro.120 male SD rats were divided into control group,LPS group and LPS + MSCs group (The above three groups were divided into 5 subgroups (2 h,8 h,24 h,48 h,96 h,n =8) according to different time points of MSCs administration);in which placebo saline,lipopolysaccharide (LPS),and LPS plus MSCs treatment were given,respectively.The inflammation factors,including TNF-α,IL-1 α and et al.,in bronchoalveolar lavage fluid,the lung wet/dry ratio,and pathological score of lung injury were compared.Results With the cells isolated,cultured and further purified,the BM-MSCs can be induced to differentiate to adipocyte-oriented,chrondrocyte-oriented and osteocyte-oriented cells.The positive rates of CD90,CD105,CD45 and CD34 were respectively 98.89％,97.37％,3.17％ and 1.41％ by flow cytometry.The maximum concentration of TNF-α,IL-1α,IL-10 were observed in 8 h subgroup of LPS group [(364.911 ± 32.182) pg/mL,(286.316 ± 18.567) pg/mL,(162.037 ± 22.377) pg/mL],and gradually decreased over time,while the maximum lung wet/dry ratio and pathological grading were observed in 2 h subgroup of LPS group(6.361 ±0.265,0.462 ± 0.048),then gradually decreased over time.As early intervention (2 h,8 h,24 h) of BM-MSCs,the inflammatory factor,dry wet ratio and pathological score were significantly different(P ＜0.05),while there were no significant difference over time (P ＞ 0.05).Conclusion Early application of BM-MSCs can reduce TNF-α and IL-1 α,so as to reduce the lung injury and alveolar edema.