Significance of N133K site polymorphism in Lp-PLA2 gene in patients with different types of coronary heart dis-eases

ZHANG Ming-ming; GAO Wei; LIU Xin; YANG Chao-ju; ZHAO Pei; YU Yue-qing; WU Hui-zhen

doi:10.3969/j.issn.1001-9448.2017.04.012

Abstract：Objective To explore the correlation and clinical significance of N 133K site polymorphism of Lp -PLA2 gene in patients with different types of coronary heart diseases (CHD).Methods From Jan 2014 to Mar 2016, data of 426 patients with angina pectoris and 415 patients with myocardial infarction were collected as case group , and data of 400 cases of physical examination were collected as control group .PCR assay combined with direct sequencing method were applied to test N133K site polymorphism of Lp-PLA2 gene.Logistic regression analysis was used to analyze the cor-relation between N133K site polymorphism of Lp-PLA2 gene and types of CHD .The concentration of serum Lp -PLA2 and lipids were assessed simultaneously .Results The serum levels of CHOL , TG, LDL-C, and Lp-PLA2 in case group were significantly higher than those of control group , and levels of these indexes in myocardial infarction patients were significantly higher than those in angina pectoris patients and control group ;while the level of HDL -C was signifi-cantly lower than that in angina pectoris patients and control group (all P<0.05).Levels of CHOL, TG, LDLC, and Lp-PLA2 in case group were significantly higher than those in control group , while the level of HDL-C was significantly (all P<0.05).After 1 month, Lp-PLA2 was significantly decreased in case group (P<0.05).The frequencies of genotype GG, GC and allele G in myocardial infarction group were significantly higher than those in angina pectoris pa -tients and control group (all P<0.05), and the frequencies of genotype GG , GC and allele G in angina pectoris group were also significantly higher than those in control group (all P<0.05).Results of Logistic regression analysis showed that genotype GG in N133K ( C/G) site of LP -PLA2 gene was significantly correlated to the severity of CHD ( P<0.01).Conclusion The N133K (C/G) site polymorphism of LP-PLA2 gene might be related to CHD.