Purification of total triterpenes from the root of Actinidia Chinensis Planch and its anti-tumor activity
WANG Qun
LEI Xin-xin
YANG Xiao-dan
XIN Tian
LI Yun-qiu
Abstract：Objective To purify total triterpenes from Actinidia Chinensis Planch root by macroporous resin and to explore its anti-tumor activity .Methods The percentage of total triterpenoids from Actinidia Chinensis Planch root was taken as indexes to determine the extraction technology by AB -8 macroporous resin .The effect of total triterpenoids on the proliferation rates of human colon cancer SW 480 cells and LOVO cells, human breast cancer MCF -7 cells, and hu-man hepatoma SMMC-7721 cells was assessed by CCK -8 assay.Cell cycle of SW480 cells was demonstrated by flow cytometry (FCM).Results The optimal conditions were as follow , the density of sample solution of 2.8 mg/mL, pH=7, flow rate of 1 BV/h, the loading volume of 4 BV, and the flow rate of 2 BV/h.The total triterpenes inhibited the pro-liferations of four tumor cells in dose -and time-dependent manners .In addition, they significantly induced cell cycle arrested at the G1 and S phase.Conclusion Using AB-8 macroporous resin would be a simple and effective method to purify total triterpenes from Actinidia chinensis Planch root.After treated with them , the proliferation of cancer cells were inhibited by inducing cell cycle arrested at the G 1 and S phase .
Keywords：Actinidia Chinensis Planchpurificationtotal triterpenoidanti-tumorcell cycle
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 514-518 )