Effects of salubrinal on LC3-ⅡmRNA and LC3-Ⅱ/LC3-ⅠmRNA in rat model of cerebral ischemia reper-fusion

LIU Li-juan; CHEN Yao; ZHOU De-sheng; WANG Hong-hai; ZHOU Ying-can; LI Zhong; HU Hua

doi:10.3969/j.issn.1001-9448.2017.04.004

Abstract：Objective To investigate the effects of salubrinal on the expression of LC 3-Ⅱ mRNA and LC3-Ⅱ/LC3-ⅠmRNA in rat model of cerebral ischemia reperfusion .Methods MCAO ischemia reperfusion model in rats were established by using thread bolt method .The rats were randomly divided into sham operation group , model group and salubrinal group (Sal group).Each group was subdivided into 4 subgroups:6 h, 12 h, 24 h, and 72 h subgroups.In each group, the neurological severity score was performed at the corresponding time points after reperfusion , and the rats were sacrificed for brain samples .HE staining and real time -PCR were performed .Results Compared with the sham operation group , the model group and the Sal group had neurological deficits at all time points ( P<0.01 ) .Compared with the model group , neurological deficits in Sal group were significantly alleviated 24 h and 72 h after reperfusion ( P<0.05).Reduced or even absence of neurons , cells swelling or ruptured, nuclear condensed with deep stained , and glial cell proliferated were observed in model group; which were alleviated after salubrinal intervention .Compared with the sham operation group , LC3-ⅡmRNA and LC3-Ⅱ/LC3-ⅠmRNA of the model group and the Sal group were all in-creased at different time points (P<0.01).Compared with the model group, the LC3-ⅡmRNA expression in Sal group was significantly decreased (P<0.05) 12 h, 24 h and 72 h after reperfusion; with LC3-Ⅱ/LC3-Ⅰ mRNA signifi-cantly reduced at each time point after reperfusion ( P<0.01 ) .Conclusion LC3-Ⅱ mRNA and LC3-Ⅱ/LC3-ⅠmRNA are reduced by salubrinal , which protects rats from injury caused by cerebral ischemia reperfusion .