The Changes and significance of the expression of TIM-1, IFN-γand IL-4 in the renal tissues of IgA ne-phropathy rats

WANG Ying; HU Gui-rong; LI Bi-min

doi:10.3969/j.issn.1001-9448.2017.04.003

Abstract：Objective To investigate the expression of T -cell immunoglobulin domain and mucin domain -1 (TIM-1), interferon-γ(IFN-γ), and interleukin-4 (IL-4) in renal tissues of IgA nephropathy rats , and to ex-plore the correlation between TIM -1 and the imbalance of IFN-γ/IL-4 and its role in the pathogenesis of IgA nephrop-athy.Methods Twenty male SD rats were randomized into 2 groups, the model group (n=10) and the control group (n=10).The pathological changes of kidney were assessed after PAS staining and immunofluorescence .Immunohisto-chemical staining was used to monitor the expression of TIM -1, IFN-γand IL-4.Results The IgA nephropathy rat model was successfully established .At the ninth weekend , 24-hour urine protein quantitation in the model group was sig-nificantly higher than that in control group (P<0.01), with significantly lower serum albumin (P<0.01).Immunofluo-rescence showed that IgA fluorescence was observed in the mesangial area of model group .PAS staining showed that the number of mesangial cells was obviously increased in glomerular of model group , with moderate to severe hyperplasia of mesangial matrix and obvious inflammatory cell infiltration in renal interstium .Katafunchi scoring in model group was sig-nificantly higher than that in control group (P<0.01).The expression quantity of TIM -1 in renal tissue of model group (7.50 ±1.58) was significantly higher than that in control group (1.02 ±0.82, P<0.01).There was no significant difference in expression of IFN -γbetween model group and control group (2.63 ±0.97 vs.2.82 ±1.03, P>0.01). The expression of IL-4 in model group was significantly higher than that in control group (7.54 ±1.58 vs.3.43 ±1.35, P<0.01).The expression of TIM-1 was significantly positively correlated with IL -4 (r=0.99, P<0.01), but signif-icantly negatively correlated with IFN -γ/IL-4 (r=-0.751, P<0.01).Conclusion TIM-1 may be involved in the occurrence and development of IgA nephropathy through regulating Th immune .