Effects and mechanism of metoprolol on the cortex oxidative stress in prefrontal lobe in rats with depression rats

WAN Yan; WANG Yi -ming

doi:10.3969/j.issn.1001-9448.2017.03.003

Abstract：Objective To observe the concentrations of NO , MAD and SOD , and the expression of eNOS in the prefrontal cortex of rats with chronic unpredictable mild stress , thus to detect the effect and mechanism of anti -depression effect of metoprolol.Methods Forty SD rats were randomly divided into 4 groups, control group, model group, model plus metoprolol group , and control plus metoprolol group .The rats were exposed to the chronic unpredictable mild stress ( CUMS) and isolation to create a depression model .Open field test and sucrose water consumption test were performed to observe the behavior of animals after intragstric administration with metoprolol ( in control plus metoprolol and model plus metoprolol groups ) or distilled water ( in control and model groups ) for 22 days.The expression of eNOS mRNA was measured with RT-PCR, as the expression of eNOS and eNOS -ser1179 protein was detected by Western blot .The con-centrations of NO , SOD and MDA were assessed by ELISA .Results Compared with the model group , the model plus metoprolol group were significantly lower in the concentrations of NO (6.12 ±1.05 μmol/mg) and MDA (8.21 ±1.25μmol/mg);and higher in concentration of SOD (55.59 ±11.07 U/mg).Compared with the model group, there was sig-nificantly higher expression in eNOS protein , eNOS mRNA and eNOS-ser1179 protein in model plus metoprolol ( P<0.05).Conclusion The metoprolol have positive effects in depression rats , which could be related with the decrease of the oxidative stress in the brain .