The effects of NR2B siRNA carring vector Tat-LK15 on chronic pain
YANG Xue
WU Hao-peng
PENG Jie
LU Jian-hua
Abstract：Objective To discuss the effects of intrathecal injection of TAT -LK15/NR2B siRNA on chronic pain relieving in vivo.Methods Adult male SD rats were randomly divided into 4 groups, Group C (control group, no treatment), Group CFA (inflammatory pain induced by freund's adjuvant complete and intrathecal injection of saline ) , Group siTat-LK15 (inflammatory pain model and intrathecal injection of Tat -LK15/NR2B siRNA) and Group ncTat-LK15 (inflammatory pain model and intrathecal injection of Tat -LK15/scrambled siRNA).The changes of NR2B ex-pression were detected using western -blot in SCDH of chronic inflammatory pain rats following intrathecal injection .Pain control efficacy was evaluated by 50% mechanical withdrawal threshold ( PWMT ) and thermal withdrawal latency (PWTL).Results NR2B protein expression was efficiently inhibited by intrathecal injection of Tat -LK15/NR2B siR-NA complexes compared with CFA group ( P<0.01 ) , while complexes injection of TAT -LK15 /scrambled siRNA did not show this inhibitory effect .Moreover, injection of Tat -LK15/NR2B siRNA complexes significantly increased PWMT and PWTL in chronic inflammatory rats compared with CFA group ( P<0.01) .Conclusion TAT-LK15 can efficiently deliver siRNA targeting NR2B in vivo and relieve chronic inflammatory pain .
Keywords：injectionsintrathecalcell penetrating peptidesgene therapytransfectionRNA small interfer-ingreceptorsN-Methyl-D-Aspartateinflammation pain
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 333-336 )