Effect of ulinastatin on hypoxia inducible factor-1αexpression of hippocampal neurons in rabbits with ischemic injury
LIU Bing-bing
ZHANG Chao-cheng
ZHOU Dai-wei
LIU Yan-jun
SHU Hai-hua
LI Na
XIAO Xiao- shan
Abstract：Objective To investigate the effects of ulinastatin on hypoxia inducible factor -1 ( HIF-1α) expres-sion of hippocampal CA 1 neurons suffering from hypoxic -ischemic injury induced by controlled hypotension ( CH) in rab-bits.Methods Thirty-six healthy male New Zealand white rabbits were randomly divided into sham operation group (Group Cont), controlled hypotension group (Group CH) and UTI pretreatment group (Group UTI+CH).The model of controlled hypotension was constructed by sodium nitroprusside .Ulinastatin (50000 U/kg) was intravenously injected 30 min before the beginning of controlled hypotension in Group UTI +CH.Western blot was applied to detect the expression of HIF-1α, Bcl-2 and Bax.The hippocampal CA1 neuron injury was observed using TUNEL staining and HIF -1αim-munohistochemical staining .Results HIF-1αand Bax protein expression of Group UTI +CH were significanlty lower than that of Group CH ( P<0.05 ) , but Bcl -2 expression was significantly higher ( P <0.05 ) .Apoptotic index of TUNEL staining was significantly higher in Group CH than in Group Cont ( P<0.05 ) , but it was significanlty lower in Group UTI+CH than in Group CH (P<0.05).Conclusion Ulinastatin maybe suppress the expression of HIF -1αand proapoptotic protein Bax , and upregulate the expression of anti -apoptotic protein Bcl -2, so as to attenuate hypoxic-is-chemic hippocampal injury induced by controlled hypotension .
Keywords：controlled hypotensionhypoxic-ischemic brain injuryulinastatinhypoxia inducible factor -1αBcl-2Bax
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 182-185 )