The increase of NF-κB (P65) expression by Miaoyao sachet in lung tissues of mice.
JIA Guo-quan
ZHANG Quan
TAN Jia-wu
WANG Hui
LIANG Ting-ting
Abstract：Objective To study the effect of Miaoyao sachet on NF -κB ( P65 ) expression in lung tissues of mice.Methods Eighty Kunming species male mice were randomly divided into 8 groups, normal control group (Group A), pidotimod group (Group C, positive control), sachet group (Group E), sachet plus pidotimod group (Group G), cold stimulation group ( Group B ) , pidotimod cold stimulation group ( Group D ) , sachet cold stimulation group ( Group F), and sachet plus pidotimod cold stimulation group (Group H).Four weeks after prophylaxis, cold water stimulation [(4 ±1)℃3 h exposure for 4 days] was performed in Groups B, D, F and H to establish immunodeficiency models .The expression of NF-κB (P65) was assessed using immunohistochemistry , quantitative real -time PCR and Western blot. Results Compared to Group A , NF-κB ( P65 ) were significantly increased in the mice bronchial epithelial cells and alveolar epithelial cells of(P<0.05).Compared to the Group B, NF-κB (P65) were significantly increased in Group D, F and H (P<0.05).Conclusion Miaoyao sachet can improve the expression of NF -κB (P65) in lung tissues of mice, strengthen the immune of respiratory tract .
Keywords：Miaoyao sachetNF-κB( P65)traditional medicine
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 178-181 )