Hepatocyte growth factor influences proliferation and invasion of non-small cell lung cancer via targeting epithe-lial-mesenchymal transition
Abstract：Objective To explore that how hepatocyte growth factor ( HGF) effects metastasis on lung cancer and its possible molecular mechanisms .Methods Non-small cell lung cancer ( NSCLC) cells were induced with HGF to established drug-resistance A549/DDP cell line.Real-time PCR and Western blot were applied to detect the expression of HGF on epithelial-mesenchymal transition(EMT)-related markers, including Vimentin and E -cadherin, which in lung cancer cell lines A549 and A549/DDP with different drug -resistance.Cell wound scratch assay and Transwell assay were applied to evaluate the effects of HGF on A 549/DDP and A549 cells′invasion and migration ability before and after the stimulation-induced.Results The expression of HGF in the lung cancer cell A 549/DDP was (2.82 ±0.23) times higher than that in A549 cells (P<0.05).Improving the level of HGF on the cellular microenvironment in A 549/DDP and A549 could stimulate the EMT makers Vimentin enhanced while E -cadherin descend , which the difference was sta-tistically significant (the genetic level P<0.01, protein level P<0.05).The cell wound scratch assay showed that the scratch width of A549/DDP group was significantly higher than that in A549 group (P<0.05).Transwell assay showed that the invasion ability of A549/DDP cells after inducing with HGF was significantly increased than A 549 group ( P<0.01).Conclusion HGF increase invasion and migration ability of A549/DDP and A549 cells via regulationg the ex-pression of EMT-related genes .
Keywords：non-small cell lung cancerhepatocyte growth factorA549A549/DDPinvasionmigrationepithelial-mesenchymal transition
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 173-177 )