Effects and mechanism of niclosamide on proliferation and apoptosis of adrenocortical carcinoma SW-13 cells

WANG Min-jie; WANG Wei; ZHU yi; ZHAO Xu; HU Wei-lie

doi:10.3969/j.issn.1001-9448.2017.01.020

Abstract：Objective To study the effects of niclosamide on cell proliferation , cell cycle, cell apoptosis, cell migration and invasion of human adrenal cortical carcinoma cells .Methods SW-13 cells were cultured in vitro .CCK-8 and iCELLigence real -time label free cell proliferation monitor were applied to detect the cell proliferation under differ-ent concentrations of niclosamide .There were control and niclosamide group .Flow cytometry was used to detect the cell cycle changes;AO/EB staining assay and annexin V/PI staining flow cytometry were applied to assess the apoptosis rate;and trans-well assay and cell scratch assay were used to assess cell invasion and migration abilities .The content changes ofβ-catenin/LRP6 were assessed using Western -Blotting.Results After treated with different concentrations of niclosamide for 24, 48, 72, 96, and 120 hours, the proliferation of SW -13 cells were significantly inhibited ( F=157.93, P<0.001), and following time and concentration dependent manners .The results of flow cytometry cycle test showed that SW-13 cell cycle were blocked in G0/G1 phase after treated with niclosamide for 24, 48 and 72 hours (P=0.001, 0.005 and 0.008), compared with the control group.AO/EB staining and Annexin V/PI staining showed that, compared with the control group , the apoptosis had significant difference after treated with niclosamide for 24 and 48 hours (P<0.001).Transwell experimental results showed niclosamide decreased SW -13 cell invasion (P<0.001), and cell scratch experiments showed that niclosamide had significant effect on the migration ability (P=0.002), and down-regu-lation in β-catenin/LRP6 of SW-13 cells ( P<0.001 ) .Conclusion Niclosamide can inhibit the proliferation , cell cycle, invasion and migration of human adrenal cortical carcinoma SW -13 cells, and promote apoptosis , which may be related to its Wnt/beta -catenin signaling pathway .It may be a new drug for the treatment of adrenal cortical carcinoma .