SIRT1 promotes the migration of lung cancer cells A549 through influencing EMT
YU Na
CHEN Jian-ming
LI Xiao-cai
ZHANG Hai-tao
WANG Ya-jun
WU Jun
ZHANG Jia-xi
Abstract：Objective To investigate the influence of SIRT1 on cell proliferation and migration in A549 cells. Methods lung cancer A549 cells were culture .The recombinant adenovirus was applied to up -regulate/down-regu-late the expression of SIRT1.MTT assay, colony formation, wound-healing assay and trans -well migration assay were performed to measure multiplication capacity and migration ability .Western Blot assay was performed to detect the influ-ence of down -regulating SIRT1 on the expression of EMT related proteins , including ZO -1, β-catenin, Snail and ZEB1.Results After up -regulating/down -regulating the expression of SIRT1, the proliferation rate of A549 cell showed no obvious change , but healing rate and migration ability of A 549 cells were enhanced/weakened .The level of EMT related epithelial markers ZO -1 was increased, while the level of interstitial markers β-catenin, Snail and ZEB1 were decreased after down -regulating the expression of SIRT1.Conclusion SIRT1 promotes lung cancer cell A549 mi-gration through influencing EMT related proteins .
Keywords：SIRT1lung cancerproliferationmigrationEMT
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 65-71 )