The Glutaminase1 expression in breast tissues of patients with breast cancer and its influence on neoadjuvant chemo-therapy

FENG De-cai; HUANG Yu-kang; WU Chu-cheng; PENG Wei-qiang; LI Hong-xing; YI Zhi-jun; HUANG Xiao-ni

doi:10.3969/j.issn.1001-9448.2016.24.013

Abstract：Objective To investigate the expression of Glutaminase 1( GLS1) gene in breast cancer and its signif-icance in neoadjuvant therapy .Methods A total of 134 patients with biopsy proven breast cancer in Huizhou People′s Hospital were included.The participants were randomly divided into 2 groups (n=67).The participants in experimental group were treated with modified radical preoperative administration of docetaxel , epirubicin plus cyclophosphamide (TEC) scheme for 3~4 cycles of chemotherapy before elected surgery .In the control group, patients received no neoad-juvant chemotherapy before surgical removal of the breast .After the operation , the tumor from two groups were treated by immunohistochemistry .Results GLS1 gene was expressed both in normal tissues and breast cancer tissues , but it was sig-nificantly increased in breast cancer tissues (P<0.05).After the application of neoadjuvant chemotherapy , the expres-sion of GLS1 was significantly lower than that in the control group (P<0.05).The expression levels of GLS1 were divid-ed from 1 to 4, and the patients was followed up according to the expression level of GLS 1.It was found that survival rate was correlated with the expression level of GLS1 (P<0.05).The higher the expression level, the lower the survival rate was.Conclusion GLS1 gene is highly expressed in breast cancer .Before modified radical mastectomy of breast cancer , the administration of adjuvant chemotherapy can significantly reduce the GLS 1 expression levels .The expression level of GLS1 gene correlates with prognosis , and the GLS1 gene can be used as a target in treatment .