The peripheral CK19 and E-cadherin expression in rats with tongue carcinogenesis

WANG Min; YANG Liu; TAN Hong; LIU Qiong-yao; LIAO Peng-cheng; NIE Min-hai

doi:10.3969/j.issn.1001-9448.2016.24.004

Abstract：Objective To evaluate the expression of serum cytokeratin 19 (CK19) and E-cadherin (E-cad) in various stages of oral carcinogenesis and investigate the correlation between CK 19 and E-cad in the process of oral cancer.Methods A total of 90 specific pathogen free ( SPF) male Wistar rats were randomly divided into two groups:4NQO-treated group (n=75,treatment of 40 parts per million [ppm] 4NQO solution) and healthy control group (n=15, treatment of water).The rats were sacrificed at different stages .The peripheral blood and tongue tissue samples were collected .The 5μm thick sections were stained with hematoxylin and eosin for histopathological analysis .Enzyme-linked immunosorbent assay ( ELISA) experimental method was used to study the expression of serum E -cad and CK19 in vari-ous stages of oral carcinogenesis .Pearson correlation coefficient and linear regression model were used to analyze the cor-relation between serum E -cad and CK19.Results The concentrations of CK19 and E-cad in normal group, epithelial hyperplasia group , mild dysplasia group , moderate-severe epithelial dysplasia group and squamous cell carcinoma group were (259.92 ±89.75) and (402.85 ±93.52) ng/L, (412.39 ±64.08) and (466.25 ±66.72) ng/L, (539.14 ± 108.07) and (593.45 ±134.06)ng/L, (784.45 ±108.53) and (848.58 ±99.68)ng/L, and (951.94 ±85.31) and (1 004.22 ±127.65)ng/L, respectively.There were significant differences between normal and epithelial hyperplasia , mild dysplasia, moderate-severe epithelial dysplasia or squamous cell carcinoma , in both E-cad and CK19 expression (P<0.05).The contents of CK19 and E-cad in peripheral blood were positively correlated (r=0.738, P<0.001). Conclusion The up-regulation expression of CK 19 and E-cad in peripheral blood is an early event in rat tongue muco-sa carcinogenesis .