Ammonia induced p38MAPK activation in astrocytes via epidermal growth factor receptor transactivation
JIANG Xue-mei
JIA Gui-zhi
CHU Ming-hui
DAI Hong-liang
Abstract：Objective To investigate the correlation between ammonia -induced activation of p38MAPK and epi-dermal growth factor receptor (EGFR) transactivation.Methods Primary astrocyte cultures were prepared and challenged by 3 mmol/L NH4 Cl to establish a hyperammonemic model .AG1478, which was the selective inhibitor of EGFR , PP1, which was the selective inhibitor of src , or GM6001, which was the selective inhibitor of matrix metalloproteinase , were added 30 min prior to ammonia stimulation.P38MAPK expression and activation were determined by Western blot .The interaction between EGFR and p38MAPK were determined by co-immunoprecipitation.Results Ammonia-induced acti-vation of p38MAPK was abolished by the pretreatment of AG 1478, the specific inhibitor of EGFR , and also by PP1, the specific inhibitor of src.This activation, however, was not affected by metalloproteinase specific inhibitor , GM6001.A similar result was seen regarding the interaction between p 38MAPK and EGFR.Conclusion Ammonia stimulation can in-duce p38MAPK activation in astrocytes via EGFR transactivation .
Keywords：ammoniaastrocytep38MAPKepidermal growth factor receptor
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3655-3658 )